Week of Experiment
7 Jan. Mon. Section: Experiment 1 - Pipetting technique and buffer preparation
Other Sections: No laboratory sessions this week.
14 Jan. Mon. Section: Experiment 2 - Determination of protein concentration by UV absorbance and Bradford assay
Other Sections: Experiment 1 - Pipetting technique and buffer preparation
21 Jan. Mon. Section: Martin Luther King Jr. Day - No classes.
Other Sections: Experiment 2 - Determination of protein concentration by UV absorbance and Bradford assay
28 Jan. All Sections: Computer Session 1 - Data graphing and curve fitting (Room 150 S. Biol. Bldg.)
All Sections: Lab report for Experiment 1 due.
4 Feb. All Sections: Computer Session 2 - Molecular modeling
11 Feb. All Sections: Experiment 3 - Enzyme kinetics; velocity as a function of enzyme concentration, titration of trypsin with BPTI.
All Sections: Lab report for Experiment 2 due.
18 Feb. Mon. Section: Presidents Day - No classes
Other Sections: Experiment 3 (cont.) - Enzyme kinetics; burst substrate titration, velocity as a function of substrate concentration, determination of Km, Vmax and kcat.
25 Feb. Mon. Section: Experiment 3 (cont.) - Enzyme kinetics; burst substrate titration, velocity as a function of substrate concentration, determination of Km, Vmax and kcat.
Other Sections: Experiment 4 - Enzyme inhibition; reversible inhibition.
4 March Mon. Section: Experiment 4 - Enzyme inhibition; reversible inhibition.
Other Sections: Experiment 4 (cont.) - Enzyme inhibition; irreversible inhibition.
Other Sections: Lab report for Experiment 3 due.
11 March Spring Break!
18 March Mon. Section: Experiment 4 (cont.) - Enzyme inhibition; irreversible inhibition.
Mon. Section: Lab report for Experiment 3 due.
Other Sections: Experiment 5 - Gel electrophoresis; preparation of reduced and alkylated ribonuclease A
25 March Mon. Section: Experiment 5 - Gel electrophoresis; preparation of reduced and alkylated ribonuclease A
Other Sections: Experiment 5 (cont.) - Gel electrophoresis; non-denaturing gel electrophoresis, trypsin digestion of ribonuclease A samples.
Other Sections: Lab report for Experiment 4 due.
1 April Mon. Section: Experiment 5 (cont.) - Gel electrophoresis; non-denaturing gel electrophoresis, trypsin digestion of ribonuclease A samples.
Mon. Section: Lab report for Experiment 4 due.
Other Sections: Experiment 5 (cont.) - Gel electrophoresis; SDS gels of samples from trypsin digestion.
8 April Mon. Section: Experiment 5 (cont.) - Gel electrophoresis; SDS gels of samples from trypsin digestion.
Other Sections: Experiment 6 - Column chromatography; Preparation and calibration of column. Also, video densitometry of gels from Experiment 5.
15 April Mon. Section: Experiment 6 - Column chromatography; Preparation and calibration of columns. Also, video densitometry of gels from Experiment 5.
Other Sections: Experiment 6 (cont.) - Column chromatography; Separation of trypsin from an inhibitor.
Other Sections: Lab report for Experiment 5 due.
22 April Mon. Section: Experiment 6 (cont.) - Column chromatography; Separation of trypsin from an inhibitor.
Mon. Section: Lab report for Experiment 5 due.
Other Sections: No lab!
29 April Finals week.
All Sections: Lab report for Experiment 6 due by 11:59 PM, Wednesday, 1 May.