Lectures are held on Tuesday and Thursday mornings from 9:40 - 10:30 AM, in Room 208 of the Crocker Science Center (CSC).
Dates Topics Suggested Reading
(from Ninfa, Ballou and Benore, 2nd Ed.)
8 Jan.
10 Jan.
Introduction to course and course mechanics
Units and measurement
pp 21-26, 30-38, 50-52 (basic lab protocols)
15 Jan.
17 Jan.
Acid-base equilibria, pH and buffers
UV-visible spectroscopy
pp 52-60
pp 65-74, 105-114
22 Jan.
24 Jan.
Dealing with uncertainties in measurement
Data analysis and curve fitting
pp 15-18
29 Jan.
31 Jan.
More on curve fitting
Introduction to proteases
5 Feb.
7 Feb.
X-ray crystallography and protein structure
Quiz no. 1 (Thursday, 8 Feb.)
12 Feb.
14 Feb.
Serine proteases
Introduction to enzyme kinetics
19 Feb.
21 Feb.
Enzyme kinetics continued pp 251-258
26 Feb.
28 Feb.
Introduction to enzyme inhibitors
Reversible enzyme inhibitors
pp 260-267
5 March
7 March
Irreversible enzyme inhibitors
No lecture on Thusday, 7 March
12 March
14 March
Spring Break!
19 March
21 March
Introduction to gel electrophoresis and thiol-disulfide chemistry
Quiz no. 2 (Thusday, 21 March)
pp 161-182
26 March
28 March
Principles of separation methods and gel electrophoresis
Non-denaturing gel electrophoresis
2 April
4 April
SDS gele electrophoresis
9 April
11 April
Introduction to chromatography
Gel filtration chromatography
pp 121-151
16 April
18 April
Other forms of chromatography
Other topics to be determined!
23 April Quiz no. 3 (cumulative)