This laboratory course is intended primarily for advanced Biology and Chemistry majors. The course focuses on methods for isolating and characterizing proteins, including chromatography, electrophoresis and enzyme kinetics. Quantitative analysis is strongly emphasized, and computers are used extensively for collecting and analyzing data.
Most of the experiments in the course utilize the well-characterized enzyme trypsin, a member of the serine protease family. Trypsin, which is illustrated above, is presently one of the best understood enzymes, and it offers many opportunities to illustrate important general principles in biochemistry.
This web page will be periodically updated as the course proceeds. Please be aware that the information provided here and in the syllabus does not represent a binding legal contract. It may be modified by the instructor when the class is given reasonable notice.