Lectures are held on Tuesday and Thursday mornings from 9:40 - 10:30 AM, in Room 208 of the Crocker Science Center (CSC).

Dates Topics Suggested Reading
(from Ninfa, Ballou and Benore, 2nd Ed.)
7 Jan.
9 Jan.
Introduction to course and course mechanics
Units and measurement, Acid-base equilibria
pp 21-26, 30-38, 50-52 (basic lab protocols)
14 Jan.
16 Jan.
More on acid-base equilibria, pH and buffers
UV-visible spectroscopy
pp 52-60
pp 65-74, 105-114
21 Jan.
23 Jan.
Dealing with uncertainties in measurement
Data analysis and curve fitting
pp 15-18
28 Jan.
30 Jan.
More on curve fitting
Introduction to proteases
4 Feb.
6 Feb.
X-ray crystallography and protein structure
Quiz no. 1 (Thursday, 6 Feb.)
11 Feb.
13 Feb.
Serine proteases
Introduction to enzyme kinetics
18 Feb.
20 Feb.
Enzyme kinetics continued pp 251-258
25 Feb.
27 Feb.
Introduction to enzyme inhibitors
Reversible enzyme inhibitors
pp 260-267
5 March
7 March
Reversible enzyme inhibitors, continued
Irreversible inhibitors
10 March
12 March
Spring Break!
17 March
19 March
Introduction to gel electrophoresis. and thiol-disulfide chemistry
Quiz no. 2 (Thursday, 19 March)
pp 161-182
24 March
26 March
Principles of separation methods and gel electrophoresis
Non-denaturing gel electrophoresis
31 March
2 April
SDS gel electrophoresis
7 April
9 April
Introduction to chromatography
Gel filtration chromatography
pp 121-151
14 April
16 April
Other forms of chromatography
Other topics to be determined!
21 April Last day of classes!
Quiz no. 3 (cumulative)