Lectures are held on Tuesday and Thursday mornings from 9:40 - 10:30 AM, in Room 208 of the Crocker Science Center (CSC).
| Dates | Topics | Suggested Reading (from Ninfa, Ballou and Benore, 2nd Ed.) |
|
|---|---|---|---|
| 8 Jan. 10 Jan. |
Introduction to course and course mechanics
Units and measurement |
pp 21-26, 30-38, 50-52 (basic lab protocols)
|
|
| 15 Jan. 17 Jan. |
Acid-base equilibria, pH and buffers UV-visible spectroscopy |
pp 52-60 pp 65-74, 105-114 |
|
| 22 Jan. 24 Jan. |
Dealing with uncertainties in measurement
Data analysis and curve fitting |
pp 15-18 | |
| 29 Jan. 31 Jan. |
More on curve fitting Introduction to proteases |
||
| 5 Feb. 7 Feb. |
X-ray crystallography and protein structure
Quiz no. 1 (Thursday, 8 Feb.) |
||
| 12 Feb. 14 Feb. |
Serine proteases Introduction to enzyme kinetics |
||
| 19 Feb. 21 Feb. |
Enzyme kinetics continued | pp 251-258 | |
| 26 Feb. 28 Feb. |
Introduction to enzyme inhibitors Reversible enzyme inhibitors |
pp 260-267 | |
| 5 March 7 March |
Irreversible enzyme inhibitors
No lecture on Thursday, 7 March |
||
| 12 March 14 March |
Spring Break! | ||
| 19 March 21 March |
Introduction to gel electrophoresis and thiol-disulfide chemistry Quiz no. 2 (Thursday, 21 March) |
pp 161-182 | |
| 26 March 28 March |
Principles of separation methods and gel electrophoresis
Non-denaturing gel electrophoresis |
||
| 2 April 4 April |
SDS gel electrophoresis | ||
| 9 April 11 April |
Introduction to chromatography Gel filtration chromatography |
pp 121-151 | |
| 16 April 18 April |
Other forms of chromatography Other topics to be determined! |
||
| 23 April | Quiz no. 3 (cumulative) |