Lectures will be held via Zoom on Tuesday and Thursday mornings from 9:40 - 10:30 AM.
| Dates | Topics | Suggested Reading (from Ninfa, Ballou and Benore, 2nd Ed.) |
|
|---|---|---|---|
| 19 Jan. 21 Jan. |
Introduction to course and course mechanics
Units and measurement, Acid-base equilibria |
pp 21-26, 30-38, 50-52 (basic lab protocols)
|
|
| 26 Jan. 28 Jan. |
More on acid-base equilibria, pH and buffers UV-visible spectroscopy |
pp 52-60 pp 65-74, 105-114 |
|
| 2 Feb. 4 Feb. |
Dealing with uncertainties in measurement
Data analysis and curve fitting |
pp 15-18 | |
| 9 Feb. 11 Feb. |
More on curve fitting Introduction to proteases |
||
| 16 Feb. 18 Feb. |
X-ray crystallography and protein structure
Quiz no. 1 (Thursday, 18 Feb.) |
||
| 23 Feb. 25 Feb. |
Serine proteases Introduction to enzyme kinetics |
||
| 2 March 4 March |
Enzyme kinetics continued | pp 251-258 | |
| 9 March 11 March |
Introduction to enzyme inhibitors Reversible enzyme inhibitors |
pp 260-267 | |
| 16 March 18 March |
Reversible enzyme inhibitors, continued Irreversible inhibitors Quiz no. 2 (Thursday, 18 March) |
||
| 23 March 25 March |
Introduction to gel electrophoresis. and thiol-disulfide chemistry | pp 161-182 | |
| 30 March 1 April |
Principles of separation methods and gel electrophoresis
Non-denaturing gel electrophoresis |
||
| 6 April 8 April |
SDS gel electrophoresis | ||
| 13 April 15 April |
Introduction to chromatography Gel filtration chromatography |
pp 121-151 | |
| 20 April 22 April |
Other forms of chromatography Other separation methods |
||
| 27 April 29 April |
Tuesday: Last day of classes! (Topics to be determined) Thursday: Final exam, 10:30 to 11:30 AM |