Lectures are held on Tuesday and Thursday mornings from 9:40 - 10:30 AM, in Room 208 of the Crocker Science Center (CSC).

Dates Topics Suggested Reading
(from Ninfa, Ballou and Benore, 2nd Ed.)
11 Jan.
13 Jan.
Introduction to course and course mechanics
Units and measurement, Acid-base equilibria
pp 21-26, 30-38, 50-52 (basic lab protocols)
18 Jan.
20 Jan.
More on acid-base equilibria, pH and buffers
UV-visible spectroscopy
pp 52-60
pp 65-74, 105-114
25 Jan.
27 Jan.
Dealing with uncertainties in measurement
Data analysis and curve fitting
pp 15-18
1 Feb.
2 Feb.
More on curve fitting
Introduction to proteases
8 Feb.
10 Feb.
X-ray crystallography and protein structure
Quiz no. 1 (Thursday, 10 Feb.)
15 Feb.
17 Feb.
Serine proteases
Introduction to enzyme kinetics
22 Feb.
24 Feb.
Enzyme kinetics continued pp 251-258
1 March.
2 March.
Introduction to enzyme inhibitors
Reversible enzyme inhibitors
pp 260-267
8 March
10 March
Spring Break!
15 March
17 March
Reversible enzyme inhibitors, continued
Irreversible inhibitors
22 March
24 March
Introduction to gel electrophoresis. and thiol-disulfide chemistry
Quiz no. 2 (Thursday, 24 March)
pp 161-182
29 March
31 March
Principles of separation methods and gel electrophoresis
Non-denaturing gel electrophoresis
5 April
7 April
SDS gel electrophoresis
12 April
14 April
Introduction to chromatography
Gel filtration chromatography
pp 121-151
19 April
21 April
Other forms of chromatography
Other topics to be determined!
26 April Other topics to be determined!
Last day of classes!
29 April Final Exam
10:30 - !1:30 AM