Lectures will be held via Zoom on Tuesday and Thursday mornings from 9:40 - 10:30 AM.

Dates Topics Suggested Reading
(from Ninfa, Ballou and Benore, 2nd Ed.)
19 Jan.
21 Jan.
Introduction to course and course mechanics
Units and measurement, Acid-base equilibria
pp 21-26, 30-38, 50-52 (basic lab protocols)
26 Jan.
28 Jan.
More on acid-base equilibria, pH and buffers
UV-visible spectroscopy
pp 52-60
pp 65-74, 105-114
2 Feb.
4 Feb.
Dealing with uncertainties in measurement
Data analysis and curve fitting
pp 15-18
9 Feb.
11 Feb.
More on curve fitting
Introduction to proteases
16 Feb.
18 Feb.
X-ray crystallography and protein structure
Quiz no. 1 (Thursday, 18 Feb.)
23 Feb.
25 Feb.
Serine proteases
Introduction to enzyme kinetics
2 March
4 March
Enzyme kinetics continued pp 251-258
9 March
11 March
Introduction to enzyme inhibitors
Reversible enzyme inhibitors
pp 260-267
16 March
18 March
Reversible enzyme inhibitors, continued
Irreversible inhibitors
Quiz no. 2 (Thursday, 18 March)
23 March
25 March
Introduction to gel electrophoresis. and thiol-disulfide chemistry pp 161-182
30 March
1 April
Principles of separation methods and gel electrophoresis
Non-denaturing gel electrophoresis
6 April
8 April
SDS gel electrophoresis
13 April
15 April
Introduction to chromatography
Gel filtration chromatography
pp 121-151
20 April
22 April
Other forms of chromatography
Other separation methods
27 April
29 April
Tuesday: Last day of classes! (Topics to be determined)
Thursday: Final exam, 10:30 to 11:30 AM