Lectures are held on Tuesday and Thursday mornings from 9:40 - 10:30 AM, in Room 208 of the Crocker Science Center (CSC).
| Dates | Topics | Suggested Reading (from Ninfa, Ballou and Benore, 2nd Ed.) |
|
|---|---|---|---|
| 10 Jan. 12 Jan. |
Introduction to course and course mechanics
Units and measurement, Acid-base equilibria |
pp 21-26, 30-38, 50-52 (basic lab protocols) | |
| 17 Jan. 19 Jan. |
More on acid-base equilibria, pH and buffers UV-visible spectroscopy |
pp 52-60 pp 65-74, 105-114 |
|
| 24 Jan. 26 Jan. |
Dealing with uncertainties in measurement
Data analysis and curve fitting |
pp 15-18 | |
| 31 Jan. 2 Feb. |
More on curve fitting Introduction to proteases |
||
| 7 Feb. 9 Feb. |
X-ray crystallography and protein structure
Quiz no. 1 (Thursday, 9 Feb.) |
||
| 14 Feb. 16 Feb. |
Serine proteases Introduction to enzyme kinetics |
||
| 21 Feb. 23 Feb. |
Enzyme kinetics continued | pp 251-258 | |
| 28 Feb. 2 March |
Introduction to enzyme inhibitors Reversible enzyme inhibitors |
pp 260-267 | |
| 7 March 9 March |
Spring Break! | ||
| 14 March 16 March |
Reversible enzyme inhibitors, continued Irreversible inhibitors |
||
| 21 March 23 March |
Introduction to gel electrophoresis. and thiol-disulfide chemistry Quiz no. 2 (Thursday, 23 March) |
pp 161-182 | |
| 28 March 30 March |
Principles of separation methods and gel electrophoresis
Non-denaturing gel electrophoresis |
||
| 4 April 6 April |
SDS gel electrophoresis | ||
| 11 April 13 April |
Introduction to chromatography Gel filtration chromatography |
pp 121-151 | |
| 18 April 20 April |
Other forms of chromatography Other topics to be determined! |
||
| 25 April | Other topics to be determined!
Last day of classes! | ||
| 1 May | Final Exam
10:30 - 11:30 AM |