Lectures are held on Tuesday and Thursday mornings from 9:40 - 10:30 AM, in Room 208 of the Crocker Science Center (CSC).
| Dates | Topics | Suggested Reading (from Ninfa, Ballou and Benore, 2nd Ed.) |
|
|---|---|---|---|
| 11 Jan. 13 Jan. |
Introduction to course and course mechanics
Units and measurement, Acid-base equilibria |
pp 21-26, 30-38, 50-52 (basic lab protocols) | |
| 18 Jan. 20 Jan. |
More on acid-base equilibria, pH and buffers UV-visible spectroscopy |
pp 52-60 pp 65-74, 105-114 |
|
| 25 Jan. 27 Jan. |
Dealing with uncertainties in measurement
Data analysis and curve fitting |
pp 15-18 | |
| 1 Feb. 2 Feb. |
More on curve fitting Introduction to proteases |
||
| 8 Feb. 10 Feb. |
X-ray crystallography and protein structure
Quiz no. 1 (Thursday, 10 Feb.) |
||
| 15 Feb. 17 Feb. |
Serine proteases Introduction to enzyme kinetics |
||
| 22 Feb. 24 Feb. |
Enzyme kinetics continued | pp 251-258 | |
| 1 March. 2 March. |
Introduction to enzyme inhibitors Reversible enzyme inhibitors |
pp 260-267 | |
| 8 March 10 March |
Spring Break! | ||
| 15 March 17 March |
Reversible enzyme inhibitors, continued Irreversible inhibitors |
||
| 22 March 24 March |
Introduction to gel electrophoresis. and thiol-disulfide chemistry Quiz no. 2 (Thursday, 24 March) |
pp 161-182 | |
| 29 March 31 March |
Principles of separation methods and gel electrophoresis
Non-denaturing gel electrophoresis |
||
| 5 April 7 April |
SDS gel electrophoresis | ||
| 12 April 14 April |
Introduction to chromatography Gel filtration chromatography |
pp 121-151 | |
| 19 April 21 April |
Other forms of chromatography Other topics to be determined! |
||
| 26 April | Other topics to be determined!
Last day of classes! | ||
| 29 April | Final Exam
10:30 - !1:30 AM |