Laboratory sessions are held from 1:00 - 5:00 PM, in Room 143 of the Crocker Science Center (CSC), except as noted..

Week of Experiment
10 Jan. Monday section: Experiment 1 - Pipetting technique and buffer preparation.
Other sections: No laboratory sessions this week.
17 Jan. Monday section: Martin Luther King Jr. Day, no classes.
Other sections: Experiment 1 - Pipetting technique and buffer preparation.
24 Jan. Experiment 2 - Determination of protein concentration by UV absorbance and Bradford assay.
31 Jan. Computer Session 1 - Data graphing and curve fitting (Room 150 S. Biol. Bldg., 2:00 PM).
Lab report for Experiment 1 due.
7 Feb. Computer Session 2 - Molecular modeling (Room 150 S. Biol. Bldg., 2:00 PM).
14 Feb. Experiment 3 - Enzyme kinetics; velocity as a function of enzyme concentration, titration of trypsin with BPTI.
Lab report for Experiment 2 due.
21 Feb. Monday section: President's day, no classes.
Other sections: Experiment 3 (cont.) - Enzyme kinetics; burst substrate titration, velocity as a function of substrate concentration, determination of Km, Vmax and kcat.
28 Feb. Monday section: Experiment 3 (cont.) - Enzyme kinetics; burst substrate titration, velocity as a function of substrate concentration, determination of Km, Vmax and kcat.
Other sections: Experiment 4 - Enzyme inhibition; reversible inhibition.
7 March Spring Break!
14 March Monday section: Experiment 4 - Enzyme inhibition; reversible inhibition.
Other sections: Experiment 4 (cont.) - Enzyme inhibition; irreversible inhibition.
Lab report for Experiment 3 due for Tues, Wed and Thurs sections.
21 March Monday section: Experiment 4 (cont.) - Enzyme inhibition; irreversible inhibition.
Lab report for Experiment 3 due for Monday section.
Other sections: Experiment 5 - Gel electrophoresis; preparation of reduced and alkylated ribonuclease A
28 March Monday section: Experiment 5 - Gel electrophoresis; preparation of reduced and alkylated ribonuclease A.
Other sections: Experiment 5 (cont.) - Gel electrophoresis; non-denaturing gel electrophoresis, trypsin digestion of ribonuclease A samples.
Lab report for Experiment 4 due for Tues, Wed and Thurs sections.
4 April Monday section: Experiment 5 (cont.) - Gel electrophoresis; non-denaturing gel electrophoresis, trypsin digestion of ribonuclease A samples.
Lab report for Experiment 4 due for Monday section.
Other sections: Experiment 5 (cont.) - Gel electrophoresis; SDS gels of samples from trypsin digestion.
11 April Monday section: Experiment 5 (cont.) - Gel electrophoresis; SDS gels of samples from trypsin digestion.
Other sections: Experiment 6 - Column chromatography; Preparation and calibration of column. Also, video densitometry of gels from Experiment 5.
18 April Monday section: Experiment 6 - Column chromatography; Preparation and calibration of column. Also, video densitometry of gels from Experiment 5.
Other sections: Experiment 6 (cont.) - Column chromatography; Separation of trypsin from an inhibitor.
25 April Monday section: Experiment 6 (cont.) - Column chromatography; Separation of trypsin from an inhibitor.
Other sections: No lab! Last day of classes: Tuesday, 21 April.
Lab report for Experiment 5 due for Tues, Wed and Thurs sections.
2 May Lab report for Experiment 5 due for Monday section.
All Sections: Lab report for Experiment 6 due by 11:59 PM, Wednesday, 4 May.