Laboratory sessions are held from 1:00 - 5:00 PM, in Room 143 of the Crocker Science Center (CSC), except as noted..
| Week of | Experiment |
|---|---|
| 10 Jan. |
Monday section: Experiment 1 - Pipetting technique and buffer preparation.
Other sections: No laboratory sessions this week. |
| 17 Jan. |
Monday section: Martin Luther King Jr. Day, no classes.
Other sections: Experiment 1 - Pipetting technique and buffer preparation. |
| 24 Jan. | Experiment 2 - Determination of protein concentration by UV absorbance and Bradford assay. |
| 31 Jan. |
Computer Session 1 - Data graphing and curve fitting (Room 150 S. Biol. Bldg., 2:00 PM). Lab report for Experiment 1 due. |
| 7 Feb. | Computer Session 2 - Molecular modeling (Room 150 S. Biol. Bldg., 2:00 PM). |
| 14 Feb. |
Experiment 3 - Enzyme kinetics; velocity as a function of enzyme concentration, titration of trypsin with BPTI.
Lab report for Experiment 2 due. |
| 21 Feb. |
Monday section: President's day, no classes.
Other sections: Experiment 3 (cont.) - Enzyme kinetics; burst substrate titration, velocity as a function of substrate concentration, determination of Km, Vmax and kcat. |
| 28 Feb. |
Monday section: Experiment 3 (cont.) - Enzyme kinetics; burst substrate titration, velocity as a function of substrate concentration, determination of Km, Vmax and kcat.
Other sections: Experiment 4 - Enzyme inhibition; reversible inhibition. |
| 7 March | Spring Break! |
| 14 March |
Monday section: Experiment 4 - Enzyme inhibition; reversible inhibition.
Other sections: Experiment 4 (cont.) - Enzyme inhibition; irreversible inhibition. Lab report for Experiment 3 due for Tues, Wed and Thurs sections. |
| 21 March |
Monday section: Experiment 4 (cont.) - Enzyme inhibition; irreversible inhibition.
Lab report for Experiment 3 due for Monday section. Other sections: Experiment 5 - Gel electrophoresis; preparation of reduced and alkylated ribonuclease A |
| 28 March |
Monday section: Experiment 5 - Gel electrophoresis; preparation of reduced and alkylated ribonuclease A.
Other sections: Experiment 5 (cont.) - Gel electrophoresis; non-denaturing gel electrophoresis, trypsin digestion of ribonuclease A samples. Lab report for Experiment 4 due for Tues, Wed and Thurs sections. |
| 4 April |
Monday section: Experiment 5 (cont.) - Gel electrophoresis; non-denaturing gel electrophoresis, trypsin digestion of ribonuclease A samples.
Lab report for Experiment 4 due for Monday section. Other sections: Experiment 5 (cont.) - Gel electrophoresis; SDS gels of samples from trypsin digestion. |
| 11 April |
Monday section: Experiment 5 (cont.) - Gel electrophoresis; SDS gels of samples from trypsin digestion.
Other sections: Experiment 6 - Column chromatography; Preparation and calibration of column. Also, video densitometry of gels from Experiment 5. |
| 18 April |
Monday section: Experiment 6 - Column chromatography; Preparation and calibration of column. Also, video densitometry of gels from Experiment 5.
Other sections: Experiment 6 (cont.) - Column chromatography; Separation of trypsin from an inhibitor. |
| 25 April |
Monday section: Experiment 6 (cont.) - Column chromatography; Separation of trypsin from an inhibitor.
Other sections: No lab! Last day of classes: Tuesday, 21 April. Lab report for Experiment 5 due for Tues, Wed and Thurs sections. |
| 2 May |
Lab report for Experiment 5 due for Monday section.
All Sections: Lab report for Experiment 6 due by 11:59 PM, Wednesday, 4 May. |