All laboratory sessions will be held via Zoom, from 1:00 - 5:00 PM.
|18 Jan.||No Lab Sessions This Week!|
|25 Jan.||Experiment 1 - Pipetting technique and buffer preparation|
|1 Feb.||Experiment 2 - Determination of protein concentration by UV absorbance and Bradford assay|
Computer Session 1 - Data graphing and curve fitting
Lab report for Experiment 1 due.
|15 Feb.||Computer Session 2 - Molecular modeling|
Experiment 3 - Enzyme kinetics; velocity as a function of enzyme concentration, titration of trypsin with BPTI.
Lab report for Experiment 2 due.
|1 March||Experiment 3 (cont.) - Enzyme kinetics; burst substrate titration, velocity as a function of substrate concentration, determination of Km, Vmax and kcat.|
|8 March||No Lab Sessions This Week!|
|15||Experiment 4 - Enzyme inhibition; reversible inhibition.|
Experiment 4 (cont.) - Enzyme inhibition; irreversible inhibition.
Lab report for Experiment 3 due.
|29 March||Experiment 5 - Gel electrophoresis; non-denaturing gel electrophoresis, trypsin digestion of ribonuclease A samples.|
Experiment 5 (cont.) - Gel electrophoresis; SDS gels of samples from trypsin digestion.
Lab report for Experiment 4 due.
|12 April||Experiment 6 - Column chromatography; Preparation and calibration of column. Also, video densitometry of gels from Experiment 5.|
Experiment 6 (cont.) - Column chromatography; Separation of trypsin from an inhibitor.
Lab report for Experiment 5 due.
|26 April||No lab! Last day of classes: Tuesday, 21 April.|
|3 May|| Finals week.
All Sections: Lab report for Experiment 6 due by 11:59 PM, Wednesday, 5 May.