All laboratory sessions will be held via Zoom, from 1:00 - 5:00 PM.

Week of Experiment
18 Jan. No Lab Sessions This Week!
25 Jan. Experiment 1 - Pipetting technique and buffer preparation
1 Feb. Experiment 2 - Determination of protein concentration by UV absorbance and Bradford assay
8 Feb. Computer Session 1 - Data graphing and curve fitting
Lab report for Experiment 1 due.
15 Feb. Computer Session 2 - Molecular modeling
22 Feb. Experiment 3 - Enzyme kinetics; velocity as a function of enzyme concentration, titration of trypsin with BPTI.
Lab report for Experiment 2 due.
1 March Experiment 3 (cont.) - Enzyme kinetics; burst substrate titration, velocity as a function of substrate concentration, determination of Km, Vmax and kcat.
8 March No Lab Sessions This Week!
15 Experiment 4 - Enzyme inhibition; reversible inhibition.
22 March Experiment 4 (cont.) - Enzyme inhibition; irreversible inhibition.
Lab report for Experiment 3 due.
29 March Experiment 5 - Gel electrophoresis; non-denaturing gel electrophoresis, trypsin digestion of ribonuclease A samples.
5 April Experiment 5 (cont.) - Gel electrophoresis; SDS gels of samples from trypsin digestion.
Lab report for Experiment 4 due.
12 April Experiment 6 - Column chromatography; Preparation and calibration of column. Also, video densitometry of gels from Experiment 5.
19 April Experiment 6 (cont.) - Column chromatography; Separation of trypsin from an inhibitor.
Lab report for Experiment 5 due.
26 April No lab! Last day of classes: Tuesday, 21 April.
3 May Finals week.
All Sections: Lab report for Experiment 6 due by 11:59 PM, Wednesday, 5 May.