Laboratory sessions are held from 1:00 - 5:00 PM, in Room 143 of the Crocker Science Center (CSC).
|6 Jan.||No laboratory sessions this week.|
|13 Jan.||Experiment 1 - Pipetting technique and buffer preparation|
|20 Jan.||Experiment 2 - Determination of protein concentration by UV absorbance and Bradford assay|
Computer Session 1 - Data graphing and curve fitting (Room 150 S. Biol. Bldg.)
Lab report for Experiment 1 due.
|3 Feb.||Computer Session 2 - Molecular modeling (Room 150 S. Biol. Bldg.)|
Experiment 3 - Enzyme kinetics; velocity as a function of enzyme concentration, titration of trypsin with BPTI.
Lab report for Experiment 2 due.
|17 Feb.||Experiment 3 (cont.) - Enzyme kinetics; burst substrate titration, velocity as a function of substrate concentration, determination of Km, Vmax and kcat.|
|24 Feb.||Experiment 4 - Enzyme inhibition; reversible inhibition.|
Experiment 4 (cont.) - Enzyme inhibition; irreversible inhibition.
Lab report for Experiment 3 due.
|9 March||Spring Break!|
|16 March||Experiment 5 - Gel electrophoresis; preparation of reduced and alkylated ribonuclease A|
Experiment 5 (cont.) - Gel electrophoresis; non-denaturing gel electrophoresis, trypsin digestion of ribonuclease A samples.
Lab report for Experiment 4 due.
|30 March||Experiment 5 (cont.) - Gel electrophoresis; SDS gels of samples from trypsin digestion.|
|6 April||Experiment 6 - Column chromatography; Preparation and calibration of column. Also, video densitometry of gels from Experiment 5.|
Experiment 6 (cont.) - Column chromatography; Separation of trypsin from an inhibitor.
Lab report for Experiment 5 due.
|20 April||No lab! Last day of classes: Tuesday, 21 April.|
|27 April|| Finals week.
All Sections: Lab report for Experiment 6 due by 11:59 PM, Wednesday, 29 April.