Laboratory sessions are held from 1:00 - 5:00 PM, in Room 143 of the Crocker Science Center (CSC).
| Week of | Experiment |
|---|---|
| 7 Jan. |
Mon. Section: Experiment 1 - Pipetting technique and buffer preparation
Other Sections: No laboratory sessions this week. |
| 14 Jan. |
Mon. Section: Experiment 2 - Determination of protein concentration by UV absorbance and Bradford assay
Other Sections: Experiment 1 - Pipetting technique and buffer preparation |
| 21 Jan. |
Mon. Section: Martin Luther King Jr. Day - No classes. Other Sections: Experiment 2 - Determination of protein concentration by UV absorbance and Bradford assay |
| 28 Jan. |
All Sections: Computer Session 1 - Data graphing and curve fitting (Room 150 S. Biol. Bldg.) All Sections: Lab report for Experiment 1 due. |
| 4 Feb. | All Sections: Computer Session 2 - Molecular modeling |
| 11 Feb. |
All Sections: Experiment 3 - Enzyme kinetics; velocity as a function of enzyme concentration, titration of trypsin with BPTI.
All Sections: Lab report for Experiment 2 due. |
| 18 Feb. |
Mon. Section: Presidents Day - No classes Other Sections: Experiment 3 (cont.) - Enzyme kinetics; burst substrate titration, velocity as a function of substrate concentration, determination of Km, Vmax and kcat. |
| 25 Feb. |
Mon. Section: Experiment 3 (cont.) - Enzyme kinetics; burst substrate titration, velocity as a function of substrate concentration, determination of Km, Vmax and kcat. Other Sections: Experiment 4 - Enzyme inhibition; reversible inhibition. |
| 4 March |
Mon. Section: Experiment 4 - Enzyme inhibition; reversible inhibition.
Other Sections: Experiment 4 (cont.) - Enzyme inhibition; irreversible inhibition. Other Sections: Lab report for Experiment 3 due. |
| 11 March | Spring Break! |
| 18 March |
Mon. Section: Experiment 4 (cont.) - Enzyme inhibition; irreversible inhibition.
Mon. Section: Lab report for Experiment 3 due. Other Sections: Experiment 5 - Gel electrophoresis; preparation of reduced and alkylated ribonuclease A |
| 25 March |
Mon. Section: Experiment 5 - Gel electrophoresis; preparation of reduced and alkylated ribonuclease A Other Sections: Experiment 5 (cont.) - Gel electrophoresis; non-denaturing gel electrophoresis, trypsin digestion of ribonuclease A samples. Other Sections: Lab report for Experiment 4 due. |
| 1 April |
Mon. Section: Experiment 5 (cont.) - Gel electrophoresis; non-denaturing gel electrophoresis, trypsin digestion of ribonuclease A samples.
Mon. Section: Lab report for Experiment 4 due. Other Sections: Experiment 5 (cont.) - Gel electrophoresis; SDS gels of samples from trypsin digestion. |
| 8 April |
Mon. Section: Experiment 5 (cont.) - Gel electrophoresis; SDS gels of samples from trypsin digestion.
Other Sections: Experiment 6 - Column chromatography; Preparation and calibration of column. Also, video densitometry of gels from Experiment 5. |
| 15 April |
Mon. Section: Experiment 6 - Column chromatography; Preparation and calibration of columns. Also, video densitometry of gels from Experiment 5.
Other Sections: Experiment 6 (cont.) - Column chromatography; Separation of trypsin from an inhibitor. Other Sections: Lab report for Experiment 5 due. |
| 22 April |
Mon. Section: Experiment 6 (cont.) - Column chromatography; Separation of trypsin from an inhibitor.
Mon. Section: Lab report for Experiment 5 due. Other Sections: No lab! |
| 29 April | Finals week. All Sections: Lab report for Experiment 6 due by 11:59 PM, Wednesday, 1 May. |