Laboratory sessions are held from 1:00 - 5:00 PM, in Room 143 of the Crocker Science Center (CSC).

Week of Experiment
6 Jan. No laboratory sessions this week.
13 Jan. Experiment 1 - Pipetting technique and buffer preparation
20 Jan. Experiment 2 - Determination of protein concentration by UV absorbance and Bradford assay
27 Jan. Computer Session 1 - Data graphing and curve fitting (Room 150 S. Biol. Bldg.)
Lab report for Experiment 1 due.
3 Feb. Computer Session 2 - Molecular modeling (Room 150 S. Biol. Bldg.)
10 Feb. Experiment 3 - Enzyme kinetics; velocity as a function of enzyme concentration, titration of trypsin with BPTI.
Lab report for Experiment 2 due.
17 Feb. Experiment 3 (cont.) - Enzyme kinetics; burst substrate titration, velocity as a function of substrate concentration, determination of Km, Vmax and kcat.
24 Feb. Experiment 4 - Enzyme inhibition; reversible inhibition.
2 March Experiment 4 (cont.) - Enzyme inhibition; irreversible inhibition.
Lab report for Experiment 3 due.
9 March Spring Break!
16 March Experiment 5 - Gel electrophoresis; preparation of reduced and alkylated ribonuclease A
23 March Experiment 5 (cont.) - Gel electrophoresis; non-denaturing gel electrophoresis, trypsin digestion of ribonuclease A samples.
Lab report for Experiment 4 due.
30 March Experiment 5 (cont.) - Gel electrophoresis; SDS gels of samples from trypsin digestion.
6 April Experiment 6 - Column chromatography; Preparation and calibration of column. Also, video densitometry of gels from Experiment 5.
13 April Experiment 6 (cont.) - Column chromatography; Separation of trypsin from an inhibitor.
Lab report for Experiment 5 due.
20 April No lab! Last day of classes: Tuesday, 21 April.
27 April Finals week.
All Sections: Lab report for Experiment 6 due by 11:59 PM, Wednesday, 29 April.