Laboratory sessions are held from 1:00 - 5:00 PM, in Room 143 of the Crocker Science Center (CSC).
| Week of | Experiment |
|---|---|
| 6 Jan. | No laboratory sessions this week. |
| 13 Jan. | Experiment 1 - Pipetting technique and buffer preparation |
| 20 Jan. | Experiment 2 - Determination of protein concentration by UV absorbance and Bradford assay |
| 27 Jan. |
Computer Session 1 - Data graphing and curve fitting (Room 150 S. Biol. Bldg.) Lab report for Experiment 1 due. |
| 3 Feb. | Computer Session 2 - Molecular modeling (Room 150 S. Biol. Bldg.) |
| 10 Feb. |
Experiment 3 - Enzyme kinetics; velocity as a function of enzyme concentration, titration of trypsin with BPTI.
Lab report for Experiment 2 due. |
| 17 Feb. | Experiment 3 (cont.) - Enzyme kinetics; burst substrate titration, velocity as a function of substrate concentration, determination of Km, Vmax and kcat. |
| 24 Feb. | Experiment 4 - Enzyme inhibition; reversible inhibition. |
| 2 March |
Experiment 4 (cont.) - Enzyme inhibition; irreversible inhibition.
Lab report for Experiment 3 due. |
| 9 March | Spring Break! |
| 16 March | Experiment 5 - Gel electrophoresis; preparation of reduced and alkylated ribonuclease A |
| 23 March |
Experiment 5 (cont.) - Gel electrophoresis; non-denaturing gel electrophoresis, trypsin digestion of ribonuclease A samples.
Lab report for Experiment 4 due. |
| 30 March | Experiment 5 (cont.) - Gel electrophoresis; SDS gels of samples from trypsin digestion. |
| 6 April | Experiment 6 - Column chromatography; Preparation and calibration of column. Also, video densitometry of gels from Experiment 5. |
| 13 April |
Experiment 6 (cont.) - Column chromatography; Separation of trypsin from an inhibitor.
Lab report for Experiment 5 due. |
| 20 April | No lab! Last day of classes: Tuesday, 21 April. |
| 27 April | Finals week. All Sections: Lab report for Experiment 6 due by 11:59 PM, Wednesday, 29 April. |