General Information
General Information
COVID-19 Information
Course Description
Instructor and TAs
Lab Sessions
Lab Safety
Lab Reports
Lectures
Text Book
Quizzes and Final Exam
Grades
Important Dates
Excused Absences
Unexcused Absences
University Policies
Faculty and Student Responsibilities
Special Accommodations
Academic Conduct
Title IX
Safety
Disclaimer
Schedules
Lab Schedule
Lecture Schedule
Resources
Syllabus (pdf)
Chemical Safety Information
Lecture Materials
Other Downloads
Internet Resources
Biol 3515/Chem 3515
Lecture Materials
The slides used in the Tuesday and Thursday lectures will be posted here, shortly after the class.
Lecture 1, 10 January
Introduction to Class
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Lecture 2, 12 January
Units, Conversion Factors, Concentration and pH
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Lecture 3, 17 January
pH and Buffers
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Lecture 4, 19 January
A Bit More on Buffers and Introduction to UV-visible Spectrophotometry
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Lecture 5, 24 January
More on UV-visible Absorbance
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Lecture 6, 26 January
Dealing with Uncertainty
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Lecture 7, 31 January
Significant Figures and Curve Fitting
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Lecture 8, 2 February
More on Curve Fitting and Introduction to Proteases
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Lecture 9, 7 February
Serine Proteases and Crystallography
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Lecture 10, 9 February
Introduction to Enzyme Kinetics
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Lecture 11, 14 February
Enzyme Kinetics, Continued
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Lecture 12, 16 February
Burst Substrates and the COVID Connection
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Lecture 13, 21 February
Notes on Quiz 1 and Estimating K
m
and V
max
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Lecture 14, 23 February
k
cat
, K
m
and Energy Profiles
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Lecture 15, 28 February
Making Insulin with Trypsin and Introduction to Enzyme Inhibitors
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Lecture 16, 2 March
Analysis of Reversible Inhibition Data and Introduction to Irreversible Inhibitors
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Lecture 17, 14 March
Irreversible Serine Protease Inhibitors
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Lecture 18, 16 March
SARS-COV2 Proteases and Inhibitors, and Introduction to Electrophoresis
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Lecture 19, 21 March
Electrophoresis and Thiol-disulfide Chemistry
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Lecture 20, 23 March
More on Disulfide Bonds, the Hydrophobic Effect and the Anfinsen Experiment
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Lecture 21, 28 March
Basic Principles of Electrophoresis
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Lecture 22, date
Native and SDS Gel Electrophoresis
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Lecture 23, 4 April
Stacking Gels and Analysis of the RNAse A Digestion Experiment
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Lecture 24, 6 April
More on Electrophoresis and Introduction to Chromatography
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Lecture 25, 11 April
Gel-filtration Chromatography
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Lecture 26, 13 April
More on Gel-filtration Chromatography and Experiment 6
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Lecture 27, 16 April
Other Chromatography Methods
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Lecture 28, 20 April
The Ultracentrifuge, Crystallography and Some History
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Lecture 29, 25 April
Cryo-Electron Microscopy and Protein Structure Prediction
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